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Testicular toxicity: evaluation during drug development

Neonatal rat ventricular myocytes (NRVMs) isolation NRVMs were isolated from 1- to 3-day-old Sprague-Dawley rats (Vital River Laboratory Animal Technology Co., Ltd, Beijing, China) using a cardiomyocyte isolation kit (Miltenyi Biotec, #130-098-373) as previously described 46

Their adducts with plasma or cell proteins can be determined after electrophoretic separation, followed by blotting

In mammals, ferroptosis has been implicated in diverse pathologies, including neurodegenerative diseases, stroke, traumatic brain injury and kidney degeneration, as well as in carcinogenesis, where it may exert a tumor-suppressor function